In Silico Functional Annotation and Structural Modeling of the Hypothetical Proteins of Halobacterium Salinarum Nrc-1
نویسنده
چکیده
Six hypothetical proteins of Halobacterium salinarum NRC-1 VNG0546c, VNG2021c, VNG2549c, VNG0683c, VNG2351c and VNG1475c were studied. Expasy’s ProtParam study of physico-chemical properties showed that these halophilic proteins are acidic in character and stable in nature having high extinction coefficient ranging from 26164.9 to 57758.3 M 1 cm–1, high aliphatic index (except in VNG221c) and low Grand Average hydropathy value. Secondary structure by SOPMA predicted alpha helix dominates among secondary structure elements followed by random coil, extended strand and beta turns for all sequences (except VNG1475c). Functional characterization was performed by prediction of motifs and conserved domains. Three dimensional structures were developed for these halophilic proteins. The modeling of the three dimensional structure of these proteins was done by PHYRE 2 sever. Protein structure checking tools PROCHECK and ProSA were used for validating the modeled structures.
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